ABSTRACT
Introdução: o câncer de mama é o tipo de câncer mais diagnosticado e a principal causa de morte entre as mulheres em todo o mundo. Aproximadamente 1,67 milhões de novos casos de câncer de mama foram diagnosticados em 2012, levando a mais de meio milhão de mortes. O câncer de mama foi responsável por 11,6% dos novos casos de cânceres diagnosticados (2.089 milhões) e 9,2% (787.000) das mortes relacionadas ao câncer para ambos os sexos e em todas as idades em todo o mundo em 2018.Objetivo: o câncer de mama como o carcinoma mais diagnosticado no mundo e a principal causa de morte entre as mulheres, é uma morbidade de grande importância, sendo o objetivo deste estudo avaliar a associação entre o polimorfismo do gene LOX G473A (rs1800449) a ocorrência de câncer de mama, potencialmente estabelecendo um novo achado na identificação de riscos, prevenção, e atendimento a um grupo específico de mulheres.Método: neste estudo de coorte retrospectivo, a frequência do polimorfismo LOX G473A foi avaliada em 148 mulheres com câncer de mama e 245 mulheres sem a doença. Todas as pacientes responderam a um questionário para identificação de possíveis fatores de risco e posteriormente realizaram coleta de sangue periférico para estudo do gene LOX. O DNA foi extraído seguido da amplificação gênica via PCR, e o polimorfismo foi estudado por eletroforese de fragmentos específicos após digestão das amostras com a endonuclease de restrição do organismo Providencia stuartii.Resultados: este estudo identificou o uso de anticoncepcional oral e o antecedente familiar de câncer de mama como fatores de risco par a doença; o polimorfismo G473A na LOX não foi identificado como fator de risco.Conclusão: não foi observada relação entre o polimorfismo LOX G473A e a ocorrência de câncer de mama.
Introduction: breast cancer is the most diagnosed type of cancer and the leading cause of death among women worldwide. Approximately 1.67 million new cases of breast cancer were diagnosed in 2012, leading to more than half a million deaths. Breast cancer accounted for 11.6% of newly diagnosed cancers (2,089 million) and 9.2% (787,000) of cancer-related deaths for both sexes and at all ages worldwide in 2018.Objective: breast cancer, as the most diagnosed carcinoma in the world and the leading cause of death among women, is a morbidity of outstanding importance, and the object of this study is to evaluate the association between the LOX gene G473A (rs1800449) polymorphism and breast cancer occurrence, potentially establishing a new finding in the identification of risks, prevention, and care for a specific group of women.Methods:in this retrospective cohort study, LOX G473A polymorphism frequency was assessed in 148 women with breast cancer and 245 women without breast cancer. All patients completed a questionnaire to identify possible risk factors and subsequently underwent peripheral blood collection to study the LOX gene. DNA was extracted followed by gene amplification via PCR, and the polymorphism was studied by specific fragment electrophoresis after digestion of the samples with the restriction endonuclease Pstl.Results: this study identified the use of oral contraceptives and family history of breast cancer as risk factors for breast cancer; the G473A polymorphism in LOX was not identified as a risk factor.Conclusion: a relationship was not observed between the LOX G473A polymorphism and the occurrence of breast cancer.
ABSTRACT
Although rare, breast cancer in males represents 1% of all cancer in men, and has shown increasing incidence in 25 years. To analyze age, gender, type of procedure performed and diagnosis of all cases of breast diseases in a public hospital in Sao Paulo, Brazil, with special focus on men. Cross-sectional study of breast surgery in 2010-2014 at Hospital Municipal Universitario de Sao Bernardo do Campo, Brazil. In the period 999 breast surgery were carried out. Patients with benign diseases were about 30 years old and patients with malignant diseases were over 50. Most surgical procedures performed on men were on the benign condition of gynaecomastia (n=21). The prevalence of breast cancer in the male population attending the public health system in Sao Bernardo was 0.27 (per 100,000). There was a single record of malignant disease in men, a 65 years old man, with histological diagnosis of ductal carcinoma. Surgical pathology of the breast in men is a rare event, and accounts for about 2.4% of mammary surgery in the municipality of São Bernardo do Campo. Awareness is necessary for health professionals and the media, regarding breast pathologies in the male population, to reduce the prejudice in the search for an early diagnosis of a condition so strongly linked to the female for the majority of people.
